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Fig. 2 | BMC Neuroscience

Fig. 2

From: Cholesterol efflux regulator ABCA1 exerts protective role against high shear stress-induced injury of HBMECs via regulating PI3K/Akt/eNOS signaling

Fig. 2

ABCA1 overexpression counteracts HSS-induced functional alterations in HBMECs. A qRT-PCR detection of the effect of ABCA1 overexpression vector on ABCA1 mRNA expression. B qRT-PCR detection of the effect of ABCA1-siRNA on ABCA1 mRNA expression. C Western blot detection of the effect of ABCA1-siRNA and expression vector on ABCA1 mRNA expression. D Densitometry analysis of western blot detection of the effect of ABCA1-siRNA and expression vector on ABCA1 protein expression. E Effect of ABCA1 on the viability of HBMECs under HSS treatment. F Effect of ABCA1 on the caspase 3/7 activity in HBMECs under HSS treatment. G Effect of ABCA1 on the apoptosis of HBMECs under HSS treatment as determined by flow cytometry. H Effect of ABCA1 on ROS production in HBMECs under HSS treatment. I Effect of ABCA1 on NO production in HBMECs under HSS treatment. J Effect of ABCA1 on the release of inflammatory cytokines in HBMECs under HSS treatment. K Effect of ABCA1 on the mRNA expression levels of inflammatory cytokines in HBMECs under HSS treatment as indicated by qRT-PCR experiment. L Effect of ABCA1 on the migration of HBMECs under HSS treatment. **p < 0.01 and ***p < 0.001 among the compared groups. Full-length blots/gels are presented in Additional file 1: Figure S3

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BMC Neuroscience

ISSN: 1471-2202

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