Fig. 6

NLRP12 overexpression averts the repressive effect of silencing LSD1 on OGD/R-induced pyroptosis of RGCs. RGC-5 cells were infected with pcDNA3.1-NLRP12 lentivirus (pc-NLRP12), with pcDNA3.1-NC lentivirus (pc-NLRP12) as the negative control. A: The protein expressions of NLRP12, NLRP3, GSDMD-N, and cleaved-Caspase1 in RGC-5 cells were examined via Western blot analysis; B: mRNA expressions of NLRP12 and Caspase 1 in RGC-5 cells determined via RT-qPCR; C: concentrations of IL-1β and IL-18 examined via ELISA; D: RGC-5 cell viability was detected via the CCK-8 method. Each cell experiment was performed in triplicate; measurement data were presented as mean ± standard deviation (SD); data in panel B (left) were analyzed via the t-test; data in panels B (right) and D were analyzed via one-way ANOVA and data in panels A and C were analyzed via two-way ANOVA, followed by Tukey’s multiple comparison test; *p < 0.05