Fig. 3

LSD1 downregulates miR-21-5p via demethylation of H3K4me2 on the miR-21-5p promoter. A: The relative enrichments of LSD1, H3K2me1, or H3K4me2 precipitated on the miR-21-5p promoter regions or the non-miR-21-5p promoter regions in OGD/R-treated RGC-5 cells were determined via the Ch-IP assay. CBB1007 (CBB) was the selective inhibitor of LSD1 and PBS treatment served as the control. B: The expression levels of H3K4me2 were examined via Western blot analysis; C: The expression levels of miR-21-5p in RGC-5 cells were detected via RT-qPCR. Each cell experiment was performed in triplicate; measurement data were presented as mean ± standard deviation (SD); data in panels B and C were analyzed via one-way ANOVA and data in panel A were verified via two-way ANOVA, followed by Tukey’s multiple comparison test; *p < 0.05