Table 2 List of antibodies used for Western blots
From: Morphine-induced changes in the function of microglia and macrophages after acute spinal cord injury
Antibody | Concentration | Manufacturer | Catalog No |
|---|---|---|---|
Unconjugated primary antibodies | Â | Â | Â |
B-arrestin | 1:100 | ThermoFisher, Waltham, MA, USA | 39-5000 |
p38 | 1:500 | Abcam, Cambridge, UK | ab31828 |
Dynorphin | 1:100 | Abcam, Cambridge, UK | ab82509 |
ERK 1/2 | 1:500 | Cell Signaling, Danvers, MA, USA | 9102S |
Kappa Opioid Receptor | 1:50 | ThermoFisher, Waltham, MA, USA | 44-302G |
| Â | 1:1000 | Â | Â |
Secondary antibodies | Â | ||
IRDye 680RD Goat Anti Mouse | 1:2000 | LI-COR Biosciences, Lincoln, NE | 926-32210 |
IRDye 800CW Goat Anti Rabbit | 1:2000 | LI-COR Biosciences, Lincoln, NE | 827-08365 |