Table 1 List of antibodies used for flow cytometry
From: Morphine-induced changes in the function of microglia and macrophages after acute spinal cord injury
Antibody | Concentration | Manufacturer | Catalog No |
|---|---|---|---|
Set 1—CD86+ Pro-inflammatory phenotype |  | ||
CD11b conjugated to biotin | 1:1000 | ThermoFisher, Waltham, MA, USA | MA5-17508 |
CD45 conjugated to eFluour450 | 1:100 | ThermoFisher, Waltham, MA, USA | 48-0461-82 |
Rabbit monoclonal to Kappa Opioid Receptor | 1:500 | Abcam, Cambridge, UK | ab183825 |
CD86 conjugated to FITC | 1:100 | ThermoFisher, Waltham, MA, USA | 11-0860-82 |
Zombie NIR (Live/Dead Stain) | 1:500 | BioLegend, San Diego, CA, USA | 423106 |
Native Streptavidin conjugated to PE/Texas Red | 1:50 | Abcam, Cambridge, UK | ab136195 |
Goat anti-rabbit Alexa 750 | 1:1000 | ThermoFisher, Waltham, MA, USA | A-21039 |
Set 2—CD206+ phagocytic phenotype |  | ||
CD11b conjugated to FITC | 1:1000 | ThermoFisher, Waltham, MA, USA | MA5-17510 |
CD45 conjugated to eFluour450 | 1:100 | ThermoFisher, Waltham, MA, USA | 48-0461-82 |
Rabbit monoclonal to Kappa Opioid Receptor | 1:500 | Abcam, Cambridge, UK | ab183825 |
CD206 conjugated to biotin | 1:50 | Antibodies Online, Germany | ABIN181884 |
Zombie NIR (Live/Dead Stain) | 1:500 | BioLegend, San Diego, CA, USA | 423106 |
Native Streptavidin conjugated to PE/Texas Red | 1:50 | Abcam, Cambridge, UK | ab136195 |
Goat anti-rabbit Alexa 750 | 1:1000 | ThermoFisher, Waltham, MA, USA | A-21039 |