Fig. 11

Quantification of Dynorphin and its precursor Pro-Dynorphin in CD11b+ cells taken from center of lesion 24-h after administration. Morphine administration significantly decreases the expression of pro-dynorphin compared to saline-treated subjects by day 7 (A) but not the expression of dynorphin (B). Western blot image (C) protein was collected from subjects that received 3 days of consecutive i.v. drug treatment. Samples taken 24-h after the last drug dose and processed with LI-COR system. (C) Western blot images were cropped to remove irrelevant sections of the image and display only the proteins of interest. Loading control: protein extracted from whole spinal cord tissue from sham subjects treated with saline (lanes 2–3). REVERT Total protein stain used as internal control. Tissue used for Western blot analysis was collected 24-h after the last drug-administration. Results shown as Mean ± S.E.M. *p < 0.05, n = 6