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Fig. 3 | BMC Neuroscience

Fig. 3

From: A circular RNA derived from GLIS3 accelerates the proliferation of glioblastoma cells through competitively binding with miR-449c-5p to upregulate CAPG and GLIS3

Fig. 3

CircGLIS3 regulates CAPG and GLIS3 in GBM cells by sponging miR-449c-5p. A Nuclear-cytoplasmic fractionation validated the subcellular position of circGLIS3. B starBase database predicted the miRNA binding with circGLIS3, CAPG, and GLIS3. C The binding sites between miR-449c-5p and circGLIS3, CAPG 3ʹUTR or GLIS3 3ʹUTR predicted on starBase and the mutant binding sites of circGLIS3, CAPG 3ʹUTR, and GLIS3 3ʹUTR were demonstrated. D RIP assay assessed circGLIS3, miR-449c-5p, CAPG, and GLIS3 in cell lysates precipitated with antibodies against IgG and Ago2. E Lysates from U251 and LN229 cells were respectively subjected to co-culturing of biotinylated circGLIS3, biotinylated CAPG 3ʹUTR, or biotinylated GLIS3 3ʹUTR in pull down assay. F, G The luciferase activity in U251 and LN229 cells co-transfected with indicated plasmids was measured to validate the binding relation between miR-449c-5p and circGLIS3/CAPG 3ʹUTR/GLIS3 3ʹUTR at the predicted sites. Student’s t-test was applied for statistical analysis in D. One-way ANOVA and two-way ANOVA were respectively applied for statistical analysis in E and in F, G. *P < 0.05, **P < 0.01

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BMC Neuroscience

ISSN: 1471-2202

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