Fig. 3

Increased expression of GSDMD in ventral horn neurons of ALS mice lumbar spinal cord. Double immunofluorescence staining showed that GSDMD (red) /NeuN (green) double-positive neurons were first observed at 60 d, 95 d of age in the ventral horn of ALS mice a, b. With disease progression, GSDMD positive signal was observe in glial cells at 108 d and 122 d of ALS mice c, d. Arrows indicate double-labelled cells. Scale bar = 50 μm