Fig. 4

Co-localization of CCL2-ir and Iba-1 labeling in cortex and CC of saline and LPS injected mice. A, B Likewise, CCL2-ir positive structures are mainly situated in perivascular regions (CCL2 panels, arrows). Delicate ramified Iba-1 labeled cells (A Iba-1 panel, arrowheads) are viewed in saline injected mice; while, in LPS injected mice, hyper-ramified and/or hypertrophic soma of Iba-1 labeled cells are encountered (B, Iba-1 panel, opened arrowheads). The co-labeled structure (B Merged, arrow-arrowhead) is usually adjacent to the vasculature like profiles either, but few. C Co-localization of CCL2-ir and Iba-1 is occasionally visualized in the CC of saline injected mice (Iba-1 Merged, inset, arrow-arrowhead), which is also adjacent to a micro-vessel like contours (framed areas and inset). D In the CC of LPS injected mice, more amoeba like Iba-1 positive somata are presented (framed areas and insets, opened arrowheads), reflecting their activated states. Some of amoeba like Iba-1 labeled cells are also CCL2-ir positive (framed area and inset, arrow-arrowhead). Some pseudopodia like structures are also CCL2-ir (CCL2 panel, arrow) and Iba-1 double positive (Iba-1 Merged panel, arrow-arrowhead). Scare bar = 100 µm in all A–D