Fig. 2

Effect of the intracerebroventricular geranylgeranylacetone injection on HSP27, pHSP27 and G6PD. a Quantitative real-time polymerase chain reaction analysis of rat cerebral cortex after ischemia–reperfusion. No statistically significant difference was observed in the heat shock protein (HSP) 27 mRNA level between groups D and G. b Immunoblotting analysis of rat cerebral cortex after ischemia–reperfusion using the indicated antibodies. The glucose 6-phosphate dehydrogenase (G6PD), HSP27, phosphorylated HSP27, and β-actin immunoblots shown were prepared from the same gel. The cropped blots are used in the figure. Full-length blots are presented in Additional files 2: Figure S1, 3: Figure S2, 4: Figure S3, 5: Figure S4. c The relative expression levels of the proteins were determined through densitometric evaluation of the immunoblots and were normalized to the expression level of β-actin. No significant difference in HSP27 expression was observed between groups D and G, but the pHSP27 protein level was significantly higher in group G than in group D. The columns represent the average of each group. The error bars are standard error of mean. A nonparametric Tukey–Kramer test was performed to compare each experimental mean (*P < 0.05; **P < 0.01; ***P < 0.001). DS: dimethylsulfoxide injection followed by sham operation group (control); GS: geranylgeranylacetone (GGA) injection followed by sham operation group; D: dimethylsulfoxide injection followed by middle cerebral artery occlusion (MCAO) and reperfusion group; G: GGA injection followed by MCAO and reperfusion group; G6PD, glucose 6-phosphate dehydrogenase; HSP: heat shock protein; pHSP: phosphorylated heat shock protein