Fig. 4

Proximity ligation assay showing pre- and postsynaptic localization of ADAM10 and BACE1 in human postmortem AD and control brain hippocampus. Human control (ctrl) (a, c, e, g, i) and AD (b, d, f, h, j) hippocampal sections were subjected to PLA and each signal (brown dot) generated denotes two proteins within 40 nm distance from each other, here ADAM10 and synaptophysin (a, b), BACE1 and synaptophysin (c, d), ADAM10 and PSD-95 (e, f) or BACE1 and PSD-95 (g, h). Primary antibodies were excluded from the negative control sections (i, j). Brain tissue and cell nuclei were visualized by a nuclear stain solution containing Mayer´s haematoxylin. Each experiment was performed at least three times (technical replicates) and representative images are shown. Scale bar 20 µm