Fig. 3

Tcf4 haploinsufficiency impairs neuronal survival, differentiation and maturation in adult neurogenesis. a Representative images and quantification of BrdU incorporating cells in the DG four weeks after last BrdU injection. Tcf4Het mice have a decreased number of BrdU + cells in DG. Scale bar, 100 µm; n = 9 and 5. b Quantification of the percentage of surviving cells normalized to number of cells that were generated four weeks before. c Representative images and quantification of the number of BrdU + single, BrdU + DCX + PROX1 + triple and BrdU + PROX1 + double positive cells four weeks after last BrdU injection. PROX1 labels granule neurons and DCX immature neurons. The number of immature neurons (BrdU + DCX + PROX1 + triple positive) is increased in Tcf4Het animals, whereas the number of mature neurons (BrdU + PROX1 + positive) is reduced. Scale bar, 50 µm; n = 9 and 5. Data are presented as mean ± SEM