Fig. 9

Characterisation of microglial changes in retinal wholemounts of Rd1 mice from postnatal day (P) 21 to P300. a–c Representative, low magnification, images of retinal wholemounts from P45 mice immunolabeled for CD68 (red) and Iba1 (green). d–l Representative, higher magnification, images of Rd1 retinas from P21, P60 and P300 immunolabeled for CD68 (red) and Iba1 (green). The morphology of Iba1-positive microglia changes from an activated amoeboid shape with retracted processes at P21 (arrowheads) to a quiescent appearance with ramified processes by P300 (arrow). Scale bars: a–c 500 μm; d–l 100 μm. m Quantification of the number of Iba1-positive and CD68-positive cells over time. Data represent mean ± SEM