Fig. 4

Effects of ISL on LPS-induced neuronal apoptosis. a Representative images show neuronal damage in the CA1 subfield and the dentate hilus of hippocampus (HP) using H&E and TUNEL staining. Scale bars = 500 μm (top row), 50 μm (middle and bottom row). b, c Histograms show the relative neuronal densities and the number of TUNEL-positive cells in the hippocampus. d–f Western blotting and histograms show the protein levels of BCL-2 and BAX and the ratio of BAX/BCL-2 in the hippocampus of each group. β-Actin was used as a loading control. Values are presented as mean ± SEM (n = 6). *p < 0.05 and **p < 0.01 vs. normal group, ^#p < 0.05 vs. LPS group