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Fig. 1 | BMC Neuroscience

Fig. 1

From: Live calcium imaging of Aedes aegypti neuronal tissues reveals differential importance of chemosensory systems for life-history-specific foraging strategies

Fig. 1

RNAseq expression, schematic representation of the GCaMP6s construct and larval fluorescence. Log2 (RPKM) expression values for the promoter, AAEL003877 (PUb) was plotted across development. Samples include, from left to right: testes; male carcasses (lacking testes); carcasses of females prior to blood feeding (NBF); female carcasses 12 h, 24 h, 36 h, 48 h, and 72 h post blood meal; ovaries from NBF females and at 12 h, 24 h, 36 h, 48 h, and 72 h post ecdysis; embryos from 0–2 h through 72–76 h; whole larvae from 1st instar, 2nd instar, 3rd instar and 4th instar; male pupae; and female pupae. A genome browser snapshot was with the Y axis showing expression level based on raw read counts of fourth instar larvae (a). A schematic representation of the piggyBac-mediated GCaMP6s construct. GCaMP6s is driven by AAEL003877(PUb)(blue) while dsRed by OpIE-2, the latter serving as a transgenic marker (b). Larval bright field images (left) and corresponding fluorescent images (right) show robust GFP transients throughout the whole body and DsRed fluorescence in the abdomen. +/+ represents wild-type larva. GCaMP6s/+/+ represents transgenic GCaMP6s larvae (c)

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