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Fig. 2 | BMC Neuroscience

Fig. 2

From: Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration

Fig. 2

Loss of Mib1 causes a reduction in glial progenitors and DRG cell death. a Transverse sections over the forelimb region of wildtype and Wnt1-Cre; Mib1fl/fl embryos at E10.5 co-immunostained for the glial marker Sox10 and the neuronal marker Isl1. b Serial section of A co-immunostained for the axonal marker Tuj1 and cell death marker TUNEL. Scale bar = 50 μm. c Quantitation of Isl1-positive and Sox10-positive cells per DRG at E10.5. Number represents mean of n = 3 embryos, with over 20 individual sections counted per embryo, ns not significant. ****p < 0.0001. d Transverse sections over the forelimb region of wildtype and Wnt1-Cre; Mib1fl/fl embryos at E11.5 co-immunostained for Sox10, Isl1 and the cell death marker cleaved-Caspase 3 (Casp3). e Serial section of D co-immunostained for Tuj1 and TUNEL. Scale bar = 50 μm. f Quantitation of Isl1-positive, Sox10-positive and Casp3-positive cells per DRG at E11.5. Number represents mean of n = 3 embryos, with over 20 individual sections counted per embryo, nt neural tube, spn spinal nerve. *p < 0.05, ***p < 0.001

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