Fig. 6
Rapamycin treatment upregulates Bcl-2 and pMAPK to promote survival during CoCl2 exposure. a Rapamycin treatment increases expression of anti-apoptotic Bcl-2 protein while reducing pro-apoptotic Bax expression. Cells were treated and analyzed as in Fig. 5a via Western blot. Left are the average relative protein expressions of Bax, and Bcl-2 in each group when normalized to β-actin protein expression. The average ratio of Bax to Bcl-2 expression was also calculated and is shown. Right is a representative image of at least three separate blots showing Bax and Bcl-2 protein expression. *p < 0.05. b The ratio of phosphorylated to unphosphorylated MAPK is increased with rapamycin treatment during CoCl2 exposure. Cells were treated and analyzed as in Fig. 2a via Western blot. Left are the average relative protein expressions of MAPK and pMAPK normalized to β-actin protein expression. Ratio was obtained by dividing relative protein expression values of pMAPK by the relative protein expression values of unphosphorylated MAPK. Right is a representative image of at least three separate blots showing MAPK and pMAPK protein expression. #p < 0.05 versus control; ##p < 0.01 versus control; **p < 0.01 versus CoCl2 alone; ***p < 0.001 versus CoCl2 alone