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Fig. 8 | BMC Neuroscience

Fig. 8

From: Limited effects of dysfunctional macroautophagy on the accumulation of extracellularly derived α-synuclein in oligodendroglia: implications for MSA pathogenesis

Fig. 8

Morphological analysis reveals small α-syn-positive inclusions however no GCI-like formation upon LC3B knockdown in oligodendroglial cells. Confocal microscopy was performed to analyze the incorporation and inclusion formation of sol and fib α-syn in oligodendroglial cells upon genetic knockdown of LC3B. Immunocytochemistry was accomplished following fixation labelling human α-syn (15G7, red), GFP ligated to the shRNA plasmid (transfection control, green) and the nucleus using DAPI (blue). α-Syn-positive inclusion were found in oligodendroglia transfected with shRNA scrambled and shRNA LC3B. No differences were detected regarding the incorporation of α-syn comparing the treatment with sol and fib α-syn. However, increased number of smaller α-syn-positive dots was detected in LC3B knockdown compared with control transfected oligodendroglia. Yet, no GCI-like accumulation was found in any of the treated cells

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BMC Neuroscience

ISSN: 1471-2202

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