Fig. 4

Oxycodone activates pro-apoptotic machinery in white matter. a Immunohistochemical analysis of phospho-eIF2α, ATF4, cleaved caspase 3 (Casp3), and NF staining in cortex area containing corpus callosum of rats treated with water (W) or oxycodone (O). Arrows point to corpus callosum. Scale bar denotes 200 μm for all images. b Immunohistochemical analysis of phospho-eIF2α, ATF4, Bax, cleaved caspase 3, and XIAP staining in corpus callosum of rats treated with water (W) or oxycodone (O). Please note that Casp3-slides were not counterstained with Hematoxyllin. Scale bar denotes 50 μm for all images. c Immunohistochemical analysis of cleaved caspase 3 and NF staining in cerebellum of rats treated with water (W) or oxycodone (O). Arrows point to Purkinjie cells enlarged in the inserts. Scale bar denotes 50 μm for the top Casp3 images and 20 μm for the middle Casp3 and lower NF images