Fig. 3

Effect of compound 3 on MAPK pathway in LPS-induced BV-2 cells. BV-2 cells were treated with LPS and with or without 5–20 µM compound 3 for 30 min. Cell lysates were prepared to evaluate the protein levels of MAPKs. The phosphorylation of p38, JNK, and ERK was measured by western blotting (a–c). Similarly, the cells were activated with LPS for 24 h as chronic activation and the expression pf MAPK was observed (d–f). The densitometric analysis of bands was performed as described in the methods section. **P < 0.01, ***P < 0.001 indicates significant differences compared with treatment with LPS alone; and ^###P < 0.001 indicates significant differences compared with untreated control group. Here Ctl indicates untreated control and LPS indicates lipopolysaccharide