Fig. 4

No gradient in immunoreactivity of other marker proteins in the SOC of NMRI mice. A: Immunohistochemistry against the inhibitory marker GlyT2 (a) and the neuronal marker NeuN (b) showed no obvious gradient in the LSO (a′, b′) or SPN (a″) of NMRI mice at P30. B: Quantification of gray values in the LSO and SPN of NMRI mice at P30 based on GlyT2 and NeuN immunoreactivity revealed no difference between lateral and medial regions in either nucleus. Bars only depict lateral values in % compared to medial. Medial values were set to 100% (dashed line). Statistical significance was tested using the Mann–Whitney U test. 15–18 ROIs were analyzed per animal and three animals per age. ns not significant