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Table 4 Matrix effects (mean ± SD) of plasma and brain on analytes (n = 5)

From: A convenient UHPLC-MS/MS method for routine monitoring of plasma and brain levels of nicotine and cotinine as a tool to validate newly developed preclinical smoking model in mouse

Concentration (ng/mL) Matrix factor (%)
Plasma
 Nicotine
  LQC 9 80.32 ± 2.46
  MQC 75 75.96 ± 18.97
  HQC 150 77.37 ± 10.84
 Cotinine
  LQC 9 90.00 ± 0.30
  MQC 225 78.47 ± 4.13
  HQC 450 87.08 ± 11.61
Brain   
 Nicotine
  LQC 9 92.61 ± 5.12
  MQC 40 91.37 ± 9.13
  HQC 80 90.54 ± 7.29
 Cotinine
  LQC 9 86.64 ± 2.38
  MQC 40 89.65 ± 8.49
  HQC 80 91.92 ± 5.16