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Table 3 (%) Recovery (Mean ± SD) of analytes and IS (n = 5)

From: A convenient UHPLC-MS/MS method for routine monitoring of plasma and brain levels of nicotine and cotinine as a tool to validate newly developed preclinical smoking model in mouse

Concentration (ng/mL) Recovery (%)
Analyte IS
Plasma
 Nicotine
  LQC 9 95.05 ± 4.37 98.20 ± 1.86
  MQC 75 103.10 ± 2.69 100.10 ± 2.36
  HQC 150 95.00 ± 8.69 100.53 ± 1.30
 Cotinine
  LQC 9 89.22 ± 4.52 98.20 ± 1.86
  MQC 225 103.42 ± 4.13 100.10 ± 2.36
  HQC 450 93.43 ± 2.49 100.53 ± 1.30
Brain
 Nicotine
  LQC 9 101.58 ± 1.00 95.79 ± 2.42
  MQC 40 94.96 ± 3.51 95.14 ± 1.03
  HQC 80 107.57 ± 6.46 95.57 ± 1.73
 Cotinine
  LQC 9 99.01 ± 5.34 95.79 ± 2.42
  MQC 40 90.37 ± 3.15 95.14 ± 1.03
  HQC 80 105.60 ± 3.71 95.57 ± 1.73