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Table 3 (%) Recovery (Mean ± SD) of analytes and IS (n = 5)

From: A convenient UHPLC-MS/MS method for routine monitoring of plasma and brain levels of nicotine and cotinine as a tool to validate newly developed preclinical smoking model in mouse

Concentration (ng/mL)

Recovery (%)

Analyte

IS

Plasma

 Nicotine

  LQC

9

95.05 ± 4.37

98.20 ± 1.86

  MQC

75

103.10 ± 2.69

100.10 ± 2.36

  HQC

150

95.00 ± 8.69

100.53 ± 1.30

 Cotinine

  LQC

9

89.22 ± 4.52

98.20 ± 1.86

  MQC

225

103.42 ± 4.13

100.10 ± 2.36

  HQC

450

93.43 ± 2.49

100.53 ± 1.30

Brain

 Nicotine

  LQC

9

101.58 ± 1.00

95.79 ± 2.42

  MQC

40

94.96 ± 3.51

95.14 ± 1.03

  HQC

80

107.57 ± 6.46

95.57 ± 1.73

 Cotinine

  LQC

9

99.01 ± 5.34

95.79 ± 2.42

  MQC

40

90.37 ± 3.15

95.14 ± 1.03

  HQC

80

105.60 ± 3.71

95.57 ± 1.73

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BMC Neuroscience

ISSN: 1471-2202

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