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Fig. 4 | BMC Neuroscience

Fig. 4

From: Myelination is delayed during postnatal brain development in the mdx mouse model of Duchenne muscular dystrophy

Fig. 4

Dystrophin-deficient oligodendrocytes have maturation delays. a Western blot analysis of dystrophin proteins in oligodendrocyte lysates at day 5 and day 8 following transfection with dystrophin (DMD) or control (ctl) siRNA, b western blot analysis of total myelin basic protein (MBP) content in lysates obtained from differentiating oligodendrocytes at indicated days post-transfection with DMD or control siRNA. Representative western blots are shown, including those for p115 as a loading control. At day 5, dystrophin knockdown oligodendrocytes have significantly less MBP than control cells (p < 0.05), however MBP levels “catch up” by day 8, c oligodendrocytes were transfected with control (ctl) or dystrophin (DMD) siRNA and differentiated for the indicated times. Immunocytochemistry was then used to visualize MBP+ oligodendrocytes. Representative images are shown, d quantification of the mean relative percent MBP+ cells (out of total cells) in oligodendrocytes transfected with control (ctl) or dystrophin (DMD) siRNA at indicated time points. At days 5 and 6, there were significantly fewer MBP+ cells within the dystrophin-deficient oligodendrocytes (p < 0.05), however by day 8 dystrophin-deficient cells were similar to control cells

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