Fig. 1

Evoked NMDA-currents in cultured hippocampal neurons are blocked by (+)-MK801 and Ro 25-6981. a Whole-cell NMDA-evoked inward currents (opening downward) induced by consecutive 5 s pulses of 100 µM NMDA + 10 µM Glycine, with a 4 min interval. The last image is an overlap of the first (black) and eighth (gray) NMDA-evoked current traces. b Inhibition of NMDA-evoked inward current by the general NMDAr antagonist (+)-MK801 (10 µM). Top shows traces of the NMDA-evoked inward currents and below the normalized area of the NMDA-evoked inward currents (−1.0 corresponding to the maximal value) for control (black circles); and before, during two consecutive exposures to (+)-MK801 and after removing (+)-MK801 (red circles). c Inhibition of NMDA-evoked inward current by the GluN2 specific blocker Ro 25-6981 (1 µM). Top shows the NMDA-evoked inward currents in a cell before, during two consecutive exposures to Ro 25-6981, and after removing Ro 25-6981. Bottom shows the normalized NMDA-evoked inward currents for control (black circles); and before and during four consecutive exposures to Ro 25-6981 (red circles). Significant different compared to the control group #p < 0.0001, +p < 0.001, two-way ANOVA with Sidak’s multiple comparison post-test; n (number of cells), control: n = 7; (+)-MK801: n = 3; and Ro 25-6981: n = 4 during the first three NMDA stimulations and 2 for the last two NMDA stimulations