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Fig. 6 | BMC Neuroscience

Fig. 6

From: Hypertonic saline attenuates expression of Notch signaling and proinflammatory mediators in activated microglia in experimentally induced cerebral ischemia and hypoxic BV-2 microglia

Fig. 6

HS and DAPT reduced the expression of inflammatory mediators Phos-NF-κB, iNOS and ROS in hypoxia-activated BV-2 microglia. a The immunoreactive bands of Phos-NF-κB (65 kDa), iNOS (130 kDa), and β-actin (42 kDa), respectively. Bar graph b, c show the expression level of Phos-NF-κB and iNOS are significantly increased in OGD BV-2 cells (*P < 0.05) and significantly depressed following treatment with 80 mM HS or 10 μM DAPT (#P < 0.05). The values represent the mean ± SD in triplicate. d The immunofluorescence of Phos-NF-κB (H, K, N, Q, red) and its co-localization within the nucleus of BV-2 cells (I, L, O, R); e The immunofluorescence of iNOS (H, K, N, Q, red); lectin+ microglia (G, J, M, P, green) and the co-localization of lectin and iNOS (I, L, O, R); f shows the immunofluorescence of ROS (G, H, I, J, green); Note that the expression of Phos-NF-κB is markedly increased and translocated to the nucleus in OGD-activated BV-2 cells and decreased with the treatment of 80 mM HS or 10 μm DAPT. The expression of iNOS and ROS is also markedly increased in parallel with Phos-NF-κB in OGD BV-2 cells and depressed significantly following treatment with 80 mM HS or 10 μm DAPT. DAPI-blue; HS hypertonic saline, DAPT N-[N-(3,5-difluorophenacetyl)-1-alanyl]-S-phenylglycine t-butyl ester, OGD oxygen glucose deprivation. Scale bars in FQ 20 μm

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