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Fig. 3 | BMC Neuroscience

Fig. 3

From: Selenium suppresses glutamate-induced cell death and prevents mitochondrial morphological dynamic alterations in hippocampal HT22 neuronal cells

Fig. 3

Superoxide accumulation measured by dihydroethidium (DHE) fluorescence. Accumulation of superoxide in the cells was measures by incubation in DHE (red color) after 24 h following glutamate and/or selenium treatments. Cells were counterstained with DAPI to label the nuclei (blue color). Relative immunointensity increased from 6.0 ± 0.8 in control to 81.0 ± 2.1 after glutamate exposure. Concurrent treatment of the cells with glutamate and selenite reduce the DHE immunointensity to 47.0 ± 1.2. F(2, 23) = 633.5. **p < 0.01 versus control and ## p < 0.01 versus glutamate by ANOVA with post hoc Tukey’s test. N = 10 in each group

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BMC Neuroscience

ISSN: 1471-2202

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