Fig. 1

Cell apoptosis and miR-34b-5p expression induced in rat hippocampus after flurothyl treatments. a Pictures of TUNEL staining (400) showed seizured mice with more cell death in both the CA1 and DG regions. DAB detection; representative of eight mice; scale bars, 100 μm. b Western blotting. Hippocampal tissue protein was extracted from control and flurothyl-treated rats at 2 h, 6 h, 1 day, 3 days, or 7 days after treatments were completed. Bcl-2, Bax, pro-caspase-3, and caspase-3 were blotted for analyses. c Bax/Bcl-2 ratio (n = 8, comparing seizured rat tissue with control rat tissue at different time points, 2 h: t ₁₄ = 8.53; 6 h: t ₁₄ = 8.33; day 1: t ₁₄ = 12.83; day 3: t ₁₄ = 10.94; day 7: t ₁₄ = 14.72; ***P < 0.0001). d Heat map summarising the patterns of expression for 72 microRNAs that were differentially expressed in hippocampal tissue of either the control or the flurothyl-treated group at one day after treatments were completed (n = 3). e Relative level of miR-34b-5p. n = 8 animals in each group (one-way ANOVA, n = 8, F _5,42 = 157.5; **1 day versus 6 h or 2 h, P < 0.01; ***1 day, 6 h or 2 h versus control P < 0.0001)