Fig. 4

Macrophages infiltration along degenerated axons in nerve intoxicated with paclitaxel. Longitudinal frozen sectioned nerve segment stained for CD68 and MBP. a MBP staining of nerve segment intoxicated with paclitaxel. The vast majority of fibers appear normal with the exception of a single fiber that show degenerated myelin (arrow). b CD68 staining. Macrophages (CD68-positive cells) infiltrate along a degenerating fiber, while not associating with other normal fibers (arrow). c Merged image of a and b. Scale bar 50 μm