Fig. 1

Characteristics of A-beta 42 peptides in vitro. a SH-SY5Y cells were treated for 24 h with A-beta 42 peptides, solvent (control) or pure culture medium (untreated). Cell viability was assessed by MTT assay (left graph) and by LDH release analysis (right graph). Values were calculated as % of control cells and are represented as mean and SD from three independent experiments (conducted in at least duplicate, n ≥ 6 as indicated). Significant differences between solvent-treated controls and A-beta 42 treated cells (one-way Anova, Bonferroni’s multiple comparisons test) are indicated by *(p < 0.05) and ***(p < 0.001). b A-beta peptides were incubated at 37 °C for 24 h under agitation in PBS (fibrillated, f). As controls, untreated A-beta 42 peptides (monomeric, m) and buffer with unrelated protein (trypsin, −) were used. 7.4 µg of protein each were subjected to 10 % SDS–polyacrylamide gel, transferred to nitrocellulose and detected with antibody 6E10 and anti-mouse horse radish-labeled secondary antibody