Fig. 6

A majority of MCT14 positive neurons are non-GAD67. Double IHC on 7-µm paraffin sections with MCT14/NeuN and MCT14/GAD67 for determination of the type of neuron MCT14 is expressed in. MCT14 is seen in red, markers in green, and DAPI is seen in blue. a Colocalization of MCT14 with NeuN, a neuronal marker, in most MCT14-expressing cells. b Colocalization of MCT14 with neurons expressing GAD67, a marker for inhibitory neurons. c A total of six (n = 2) double IHC images with MCT14/NeunN and MCT14/GAD67 from retrosplenial cortex, hypothalamus and piriform cortex were collected and analyzed using a colocalization analysis pipeline from CellProfiler.org (Additional file 1: Figure S1). Fraction of MCT14-positive cells that also expressed respective marker was plotted. All images were acquired on a Zeiss AxioImager at ×20 magnification. Statistics were performed with Students’ t test, *p < 0.05