Fig. 5

The mRNA expression of HIF-1α and the number of HIF-1α positive cells after reperfusion. a The expression of HIF-1α mRNA. The level of HIF-1α in I/R group was much higher than in sham group at 1 day post operation (^#p < 0.05). But after LRIP administration, the level of HIF-1α was much lower than I/R group (*p < 0.05). At 3 days and 7 days, LRIP group still had a lower mRNA level of HIF-1α than I/R group, but the difference was not statistical significance. b The change of HIF-1α protein at 3 days. The upper one shows the western blot of β-action and HIF-1α. The below one is the quantitative analysis. LRIP suppressed the protein level of HIF-1α (^#p < 0.05), which was upregulated by cerebral ischemia injury (*p < 0.05)