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Table 3 Primer pairs used in the characterization of 3′ end of wdr81

From: Characterization of a novel zebrafish (Danio rerio) gene, wdr81, associated with cerebellar ataxia, mental retardation and dysequilibrium syndrome (CAMRQ)

  Primer Primer sequence Expected amplicon size (bp)
Primer pair 1 F (Pair1F_Drwdr81_3RACE) 5′-CTGACAACGGTGCCATCAGG-3′ 717
R (Pair1R_Drwdr81_3RACE) 5′-TTCAGGACCATCCCATTGCATA-3′
Primer pair 2 F (Pair2F_Drwdr81_3RACE) 5′-CTGTATCCACGTCAATGGAGCGTAA-3′ 757
R (Pair2R_Drwdr81_3RACE) 5′-GAAGCATTGTTCAATGTACGTTCGGTA-3′
Primer pair 3 F (Pair3F_Drwdr81_3RACE) 5′-CATTTATGGTTCGCTAATTCCCTCAA-3′ 634
R (GeneRacer™ 3′ Primer) 5′-GCTGTCAACGATACGCTACGTAACG-3′