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Fig. 10 | BMC Neuroscience

Fig. 10

From: Characterization of a novel zebrafish (Danio rerio) gene, wdr81, associated with cerebellar ataxia, mental retardation and dysequilibrium syndrome (CAMRQ)

Fig. 10

Detection of the insertion in the tested developmental and tissue samples except kidney, intestine, gills. a Agarose gel electrophoretic separation of the amplicons as products of a PCR experiment in which the presence of the detected insertion site in wdr81 3′ UTR was tested among templates from several developmental stages (top gel picture). Presence and integrity of the cDNA samples were tested with beta-actin amplification (lower gel picture). b Agarose gel electrophoretic separation of the amplicons as products of a PCR experiment in which the presence of the detected insertion site in wdr81 3′ UTR was tested among templates from several adult tissues (top gel picture). Presence and integrity of the cDNA samples were tested with beta-actin amplification (lower gel picture). In all gels, lanes were labelled with the cDNA source of each reaction loaded. Lane indicated as M was loaded with pUC mix DNA Marker (SM0301, Thermo Scientific). “No t.” indicates the no template negative control sample in which water was used instead of a template

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BMC Neuroscience

ISSN: 1471-2202

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