|
NMR
|
|---|
|
Metabolite
|
Fold change in Mras
−/− urine
|
|---|
|
Glycerate
|
+15.2
|
|
Methionine
|
+6.5
|
|
Ascorbate
|
+4.1
|
|
N-Carbamoyl-β-alanine
|
+3.9
|
|
N,N-Dimethylglycine
|
+3.1
|
|
Hypoxanthine
|
+2.8
|
|
Isoleucine
|
+2.6
|
|
Betaine
|
+2.6
|
|
Tyrosine
|
+2.5
|
|
2-Oxcisocaproate
|
+2.2
|
|
Trimethylamine
|
+2.0
|
|
Succinate
|
-4.8
|
|
1-Methlynicotinamide
|
−2.5
|
|
N-Phenylacetylglycine
|
−2.4
|
|
Hippurate
|
−2.2
|
|
Acetoacetate
|
−2.0
|
|
DI-MS
|
|---|
|
Metabolite group
|
# Metabolites analyzed
|
Increased >2-fold in Mras
−/− urine
|
Decreased >2-fold in Mras
−/− urine
|
|---|
|
Acyl carnitines
|
40
|
1 (2.5 %)
|
1 (2.5 %)
|
|
Phosphatidylcholines
|
59
|
38 (64.4 %)
|
5 (8.5 %)
|
|
Lysophosphatidylcholines
|
7
|
3 (42.9 %)
|
1 (14.3 %)
|
|
Sphingomyelins
|
9
|
1 (11.1 %)
|
4 (44.4 %)
|
- WT and Mras
−/− male urine samples were analyzed by NMR and direct-injection mass spectrometry (DI-MS). Total number of metabolites analyzed by NMR: 65; DI-MS: 116. Some differences in relative metabolite quantities were confirmed by gas chromatography (GC)-MS analysis: Glyceric acid: +13.5; l-tyrosine: +2.41; succinic acid: −3.85; hippuric acid: −2.17 (fold-change in Mras
−/− urine)
