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Table 1 Urine metabolomics analysis

From: Absence of M-Ras modulates social behavior in mice

NMR
Metabolite Fold change in Mras / urine
Glycerate +15.2
Methionine +6.5
Ascorbate +4.1
N-Carbamoyl-β-alanine +3.9
N,N-Dimethylglycine +3.1
Hypoxanthine +2.8
Isoleucine +2.6
Betaine +2.6
Tyrosine +2.5
2-Oxcisocaproate +2.2
Trimethylamine +2.0
Succinate -4.8
1-Methlynicotinamide −2.5
N-Phenylacetylglycine −2.4
Hippurate −2.2
Acetoacetate −2.0
DI-MS
Metabolite group # Metabolites analyzed Increased >2-fold in Mras / urine Decreased >2-fold in Mras / urine
Acyl carnitines 40 1 (2.5 %) 1 (2.5 %)
Phosphatidylcholines 59 38 (64.4 %) 5 (8.5 %)
Lysophosphatidylcholines 7 3 (42.9 %) 1 (14.3 %)
Sphingomyelins 9 1 (11.1 %) 4 (44.4 %)
  1. WT and Mras / male urine samples were analyzed by NMR and direct-injection mass spectrometry (DI-MS). Total number of metabolites analyzed by NMR: 65; DI-MS: 116. Some differences in relative metabolite quantities were confirmed by gas chromatography (GC)-MS analysis: Glyceric acid: +13.5; l-tyrosine: +2.41; succinic acid: −3.85; hippuric acid: −2.17 (fold-change in Mras / urine)