Skip to main content
Fig. 4 | BMC Neuroscience

Fig. 4

From: Generation of a novel monoclonal antibody that recognizes the alpha (α)-amidated isoform of a valine residue

Fig. 4

Identification of valine amide antibodies in mice. Animals defined as R1–R7 (female BALB/c mice, 8–9 weeks, n = 7) were vaccinated with an initial subcutaneous injection with a 1:1 emulsion of 50 µg of keyhole limpet hemocyanin (KLH)-Val-CONH2 immunoconjugate and complete Freund’s adjuvant, followed by booster injections with incomplete Freund’s adjuvant. Sera were collected and used to identify high-titer valine amide antisera (VAA) in the vaccinated animals using a standard ELISA. The wells were coated with synthetic immunoconjugates of bovine serum albumin (BSA)-[Tyr0]-Gly2–4-Val-CONH2 or BSA-RGDV-COO. The BSA-RGDV-COO-coated wells displayed no significant signal (data not shown, see text for additional details). Assays were performed in triplicate using two serial dilutions of mouse antisera (1:100; 1:1000). The ordinate describes the immunoreactive signals detected as absorbance at 490 nm (A490) in the assay. The values are expressed as the mean ± SEM

Back to article page
\