Fig. 1

Dcx is up-regulated upon Dicer deletion in adult neural stem cells. a–c The samples analyzed were adult neural progenitor cells (NPCs) Dicer flox/flox (f/f) or Dicer −/−. Dicer deletion was obtained by transducing Dicer f/f cells with HTN-Cre protein. a Taqman quantitative RT-PCR of miR125b and let7-c mature miRNA transcripts. Transcript levels were normalized against the housekeeping gene snoRNA55 and calculated respect to Dicer f/f (control) that is set to 1. n = 3, bars represent the mean ± standard error of the mean. b Quantitative RT-PCR of the indicated transcripts. Transcript levels were normalized against the housekeeping gene Srp14 and calculated respect to Dicer f/f (control) that is set to 1. The blue horizontal bar indicates the scale change in the y-axis. n = 3, bars represent the mean ± standard error of the mean. c Western blot showing a representative picture of the Dicer, Dcx and Actin (loading control) protein levels in NPCs (P) or differentiating NPCs (D; 4 days after growth factor withdrawal) of the indicated genotype. Most of the Dicer −/− cells die upon induction of differentiation and therefore are not included in the analysis. Three independent biological samples have been analyzed. Shown are representative pictures