Fig. 5

UbcM2 expression is not altered in Nrf2-deficient brains. A Average expression of UbcM2 in whole brain, consisting of the analysis of 20 substructures, indicating no change in aged, Nrf2-deficient brain relative to aged, strain-matched controls. n = 5 in triplicate. B Western blot analyses of Nrf2 knockout and age/strain-matched wt mice to detect markers of proteotoxicity and stress. Antibodies used are listed to the right of each panel, and the age and genotype of the mice are indicated above each lane. The positive control (+ctrl) for HSP70 induction and the accumulation of polyubiquitylated proteins is from retinal pigment epithelium (RPE-1) cells treated for 8 h with 10 µm proteasome inhibitor MG132 and harvested with 2 times concentrated Laemmli buffer. The vertical line between lanes 7 and 8 represents a lane in which molecular weight markers were run. The migration of these markers is indicated to the left. C Representative photomicrographs of dentate gyrus (a, b) and cerebellum (c, d) from aged Nrf2-deficient (92 week; b, d) and strain-matched control (98 week; a, c) brains immunolabeled with an α-ubiquitin antibody