Fig. 3

Neurons express UbcM2 at relatively high levels in hindbrain and low levels in caudate putamen. A Representative images of 7 μm, paraffin-embedded sagittal brain sections from a 4-month old C57BL/6 mouse. Anatomical regions shown are pontine nuclei (a–d), hippocampal CA1 (e–h), cingulate cortex (i–l), and caudate putamen (m–p). DNA is counterstained with Hoechst (white), and sections are immunostained for the nuclear neuronal marker NeuN (green) and UbcM2 (red). Co-localization of NeuN and UbcM2 is indicated by yellow in the merged images (d, h, l, p). 20×, size bar 30 μm. B Brain substructure-specific UbcM2 expression as measured by fluorescence intensity relative to the area of highest expression (spinal trigeminal nucleus). Error bars indicate 95 % confidence intervals. Disjoint confidence interval bars identify regions with significantly different UbcM2 levels