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Figure 2 | BMC Neuroscience

Figure 2

From: Effect of full flavor and denicotinized cigarettes exposure on the brain microvascular endothelium: a microarray-based gene expression study using a human immortalized BBB endothelial cell line

Figure 2

Effects of CSE on Phase I detoxification genes. HCMEC/D3 cells were exposed to nicotine or CSE from different products (see “Methods”). Effects of CSE and nicotine on NQO-1 mRNA and protein expression were characterized by: a1 gene array analysis; a2 real-time RT-PCR; b immunofluorescence and western blot analyses of NQO-1 protein. Representative western blots were shown with actin as a loading control. Data were expressed as mean ± SEM (fold change over control). *p < 0.05, **p < 0.01, ****p < 0.0001 vs. control. n = 6/biological replicates/condition.

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