Skip to main content
Figure 3 | BMC Neuroscience

Figure 3

From: Targeted inhibition of the Shroom3–Rho kinase protein–protein interaction circumvents Nogo66 to promote axon outgrowth

Figure 3

Inhibition of the Shroom3–ROCK protein–protein interaction with CCG-17444 enhances neurite outgrowth. P19-derived neurons were treated with 25 μM CCG-17444 or vehicle control (DMSO) for 24 h. a, b CCG-17444 enhances neurite length in neurons transfected with an RNAi control vector. Neurons transfected with POSH RNAi or Shroom3 RNAi vectors exhibit enhanced neurite length due to reduction of POSH or Shroom3 function but CCG-17444 does not further enhance neurite length, suggesting that CCG-17444 selectively targets the POSH/Shroom3 signaling complex (n = 3). Student’s t test: *p < 0.0001, Control RNAi vs CCG-17444 treated Control RNAi; #p < 0.0001, Control RNAi vs Shroom3 or POSH RNAi; **p < 0.0005, CCG-17444 Control RNAi vs CCG-17444 Shroom3 RNAi; +p < 0.0001, CCG-17444 Control RNAi vs CCG-17444 POSH RNAi. Scale bar 100 μm.

Back to article page

BMC Neuroscience

ISSN: 1471-2202

Contact us