Stress-induced increases in TNFα, IL-17A, and IL-10. Verification of multiplex measurements of (A) TNFα, (B) IL-17A, and (C) IL-10 by ELISA. Plasma levels of TNFα, IL-17A, and IL-10 were measured by ELISA in mice treated with inescapable foot shocks stress once or two times separated by 24 hr. Values are means ± SEM (n = 3–5 mice/group). Data were analyzed by one-way analysis of variance (ANOVA) followed by Bonferroni post-test using Prism software.*p < 0.05 compared to control [Ctl]).