Figure 3

Effects of GPS and GP-EX on L-DOPA-induced AIMs in 6-OHDA-lesioned rats. L-DOPA (25 mg/kg, i.p.) and benserazide (15 mg/kg, i.p.) treatment was started 6 weeks after the 6-OHDA lesion once a day for 22 days. Either GPS (25 and 50 mg/kg, p.o.) or GP-EX (50 mg/kg, p.o.) was given 30 min prior to L-DOPA treatment once a day for 22 days. The AIMs were scored for 1 min every 20 min for total 180 min after L-DOPA treatment on the indicated day according to the description in the Materials and methods section. A: axial AIMS; B: limb AIMs; C: orolingual AIMs; D: locomotive AIMs. The results are expressed as mean ± S.E.M. for 8–10 animals/group. –■–, saline treatment; −□–, L-DOPA; −▲–, L-DOPA + GP-EX (50 mg/kg); −◆–, L-DOPA + GPS (25 mg/kg); −○–, L-DOPA + GPS (50 mg/kg). ^* P < 0.05 compared with saline-treated group at each treatment day; ^# P < 0.05 compared with L-DOPA alone-treated group at each treatment day (non-parametric Kruskal-Wallis one-way ANOVA test); ^† P < 0.05 compared with the score of day-1 (Friedman repeated measures ANOVA test).