QPCR confirmations of the differential expressions of selected lncRNAs in METH-sensitized mice. A, Differentially expressed lncRNAs as detected by ssRNA-seq. 12 significantly altered lncRNAs are shown with the corresponding RPKM. # indicates the following significance cutoff: P < 0.001, FDR ≤ 0.0001 and an absolute fold change value ≥1.25. B, Validation of differentially expressed lncRNAs by qPCR. The expressions of the 12 selected lncRNAs were detected by qPCR. The expression levels were calculated relative to GAPDH. The values are presented as the means ± the SEMs. The differences between the saline and METH groups were statistically tested with independent-sample t-tests. *P < 0.05 compared to saline, n = 11-15. C, The ssRNA-seq and qPCR correlations are shown above. Pairwise scatterplots comparing the fold changes (log2 METH/saline) of the selected lncRNAs in the NAc as computed from the ssRNA-seq data (horizontal axis) and the qPCR data (vertical axis). The Pearson’s Coefficient is represented as the linear correlation coefficient, r.