Figure 2

Representative photomicrograph of silver grains on TrkB mRNA+ cells in the rhesus macaque hippocampus. Top panel: Acetylcholinesterase stained coronal section of the hippocampal formation. The dark brown band of tightly packed cells is the granule cell layer of the dentate gyrus (GCL). The subgranular zone (SGZ) is the ~40 μm wide band underneath the GCL. The approximate location of the boundary between the SGZ and hilus is indicated with a black line. (A) Localisation of TrkB-TK+ mRNA+ cells. Scale bar = 500 μm. (B-C) The majority of TrkB-TK+ mRNA+ cells were found more than 40 μm beneath the dentate gyrus into the hilus (arrows). A limited number of TrkB-TK+ mRNA+ cells were found within the subgranular zone (arrowheads). Scale bar = 100 μm. (D) Localisation of TrkB-TK- mRNA+ cells. Scale bar = 500 μm. (E-F) TrkB-TK- mRNA+ cells are concentrated within the subgranular zone (arrows). A limited number of cells on the outer edge of the granule cell layer are also TrkB-TK- mRNA + (arrow heads). Scale bar = 100 μm.