Stimulatory effect of NGEN on BK
-channel activity measured from α -hSlo -expressing HEK293T cells. (A) Original current traces showing the activity of BKCa channels before (left) and after application (right) of 30 μM NGEN. Inside-out recordings were conducted with symmetrical K+ concentration (145 mM). The potential was constantly held at +60 mV, and bath medium contained 0.1 μM Ca2+. Channel openings give an upward deflection in current. (B) Summary of the data showing effect of NGEN and NGEN plus verruculogen (Verr; 1 μM) on the probability of BKCa-channel openings (mean ± SEM; n = 8-11 for each bar). *Significantly different from control. **Significantly different from NGEN (30 μM) alone group.