Figure 5

Absence of NP1 protein in NP1-KO OGD conditioned medium does not cause neuronal death in WT normoxia cortical cultures. Morphological evidence of degenerated cell bodies and processes reveal that WT OGD CM induced neuronal degeneration in control WT cultures (A- A1) whereas, OGD conditioned NP1-KO CM did not cause cell death in WT cultures (B- B1). In contrast, NP1-KO cortical cultures, which are protected against OGD, showed neuronal death when treated with WT-OGD CM (C- C1). Representative light microscopic images are shown, n = 6, Scale, 100 μm.