Three-dimensional (3-D) view of co-localization of Gβγ and microtubules (MTs). Co-localization of overexpressed Gβγ (green) with MTs (red) as visualized by high-resolution 3-D confocal images using Volocity software (see Methods). The images shown in this assembly are still frames from Additional file 4: Movie 1 (Supplementary materials). (A) A still frame from the movie separated into its component channels: MT (red) and Gβγ (green) expression are each confined discretely to similar subcellular locations as shown in the merged panel (yellow). (B) Representative still frames were selected to summarize the movie content. The numbers on the top right of each still image denote the frame numbers within the movie. Arrows in frame 819 correspond to MT expression (red, top arrow) and Gβγ (green, bottom arrow) expression. The arrow in frame 866 points to co-localization of MT and Gβγ (yellow). The edges of each individual square in the background grid for each image are 19.21 μm in length. For detailed description, please see the text.