Table 2 Sequences, anealing temperatures, efficiencies and amplicon lengths of primers used for qPCR
From: Social stress increases expression of hemoglobin genes in mouse prefrontal cortex
Gene Name | Forward or reverse primer | Primer sequence | Anealing temperature | PCR efficiency (%) | Amplicon lengths |
|---|---|---|---|---|---|
1500015O10Rik | F | TGGGTCCAGATGGCATAAGTGG | 60 | 82** | 105 |
R | TTGCTGTGTTCTCGGCTACAG | ||||
Agxt2l1 | F | GCTCTCCGTTTGCTACTTCAC | 60 | 83* | 182 |
R | CCCTCTTGACATCTTTGCCCTT | ||||
Fam107a | F | CGCTGGTCAGTGTGGTGATT | 62 | 97* | 206 |
R | AGAGCACCGTCGCAGGAAT | ||||
Hbb-b1 | F | CTGATTCTGTTGTGTTGACTTG | 60 | 87** | 188 |
R | AGGTCTCCAAAGCTATCAAAGT | ||||
Mgp | F | ACCCTGTGCTACGAATCTCAC | 60 | 95** | 140 |
R | TTGTTGCGTTCCTGGACTCT | ||||
Tbp | F | GCAGTGCCCAGCATCACTATT | 60 | 93*,102** | 162 |
R | AAGCCCTGAGCATAAGGTGG |