Figure 1

Abnormal expression of Npc1 protein, the number of apoptotic cells and defective retinal ganglion cells in the Npc1 ^-/-retina. Retinal sections from postnatal day (P) 65 Npc1^+/+ and Npc1^-/- mice were immunostained with antibodies against Npc1 (A, B, D, E), NeuN (G-L), SMI32 (M-R), and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay (white in C, F). Nuclei were stained with DAPI (blue). I, L, O, R show the magnification of the inner part of retina from H, K, N, Q, respectively. Abbreviations: ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bar: 5 μm in O for O, R; 10 μm in I for I, L and in M for M, N, P, Q; 50 μm in A for A, B, D, E, G, H, J, K and in C for C, F.